Dr. Ralph Jimenez provides an overview of Live Cell Fluorescence Imaging. Fluorescence imaging is a widely used method for resolving molecular events in cells and tissues. The development of genetically-encoded fluorophores and a detailed understanding of their photophysics is critical for quantitative bioimaging. Dr. Jimenez provides an overview of the properties of fluorescent proteins and biosensors and describes some of his research to quantify and improve the performance of these imporatnt biological probes. Dr. Jimenez also describes a new research direction aiming to dramatically enhance bioimaging by employing quantum optics techniques.